Assessment of Genotoxic Effects of Pendimethalin in Chinese Hamster Over Cells by the Single Cell Gel Electrophoresis (Comet) Assay

Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay

Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...

Calibration of the single cell gel electrophoresis assay, flow cytometry analysis and forward mutation in Chinese hamster ovary cells.

The induction kinetics of genetic damage were measured in one clone of a mammalian cell line (CHO AS52) with three genotoxicity assays, the single cell gel electrophoresis (Comet) assay, laser beam flow cytometry and forward mutation. The first two assays allow for the rapid analysis of genotoxic damage in individual nuclei. The alkaline Comet assay detects DNA strand breaks, alkali-labile site...

Single Cell Gell Electrophoresis (Comet Assay)

DNA Damage Induced by Tartrazine in rat whole blood using Comet Assay (Single Cell Gel Electrophoresis)

Tartrazine is an orange-colored, water soluble powder widely used in food products, drugs and cosmetics. The acceptable daily intake (ADI) oh human is 0-7.5 mg/kg body weight. The objective of our study was to examine the genotoxic effect of different Tartrazine concentrations of fresh whole blood cells; by using alkaline single-cell gel electrophoresis (SCGE), also called the comet assay. This...

Assessment of DNA damage induced by butachlor on Cyprinuscarpio(L. 1758) using Single Cell Gel Electrophoresis

Butachlor (N-(butoxymethyl)-2-chloro-N-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in Asian region, especially in the north of Iran. Several studies have been reported on toxicity of butachlor, but little information is accessible on DNA damaging potential. Thus, the present study was undertaken to investigate of DNA damage by butachlor in Cyprinuscarpio(Pisces: Cyprini...